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CHAPS-RO

Roche

CHAPS

98% (from N), suitable for dialysis, solubility: >50% (Aqueous solutions)

Synonym(s):

CHAPS, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, 3-[(3-cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate, zwitterionic, Cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, zwitterionic, cholamidopropyl-dimethyl-ammonio]-1-propane sulfonate, 3-3-, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
Molecular Weight:
614.88
MDL number:
UNSPSC Code:
12161900

description

zwitterionic

Assay

98% (from N)

form

crystals

mol wt

micellar avg mol wt 6150
614.88 g/mol (anhydrous basis)

packaging

pkg of 10 g (10810118001)
pkg of 50 g (10810126001)

manufacturer/tradename

Roche

aggregation number

10

technique(s)

dialysis: suitable

CMC

4 mM
6 mM (20-25°C)

transition temp

cloud point >100 °C

solubility

>50% (Aqueous solutions)

absorption

<0.06% at 278 nm in water at 1 % (w/v) (Ease of removal*: ++
Note:
* Arbitrary scale ranges from ++ to −)

storage temp.

2-8°C

SMILES string

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

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General description

3-[(3-Cholamidopropyl)dimethyl-ammonio]-1-propane sulfonate

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Solubilization of membrane proteins. Combines the properties of both the sulfobetaine-type and bile-acid detergents.

Physical form

Crystals

Preparation Note

Working concentration: PAGE: 6.5 to 13 mM
Storage conditions (working solution): Do not store in solution, because precipitation may occur.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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