HGAMMAG-303E
MILLIPLEX® Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.
Synonym(s):
Human IgE Bead Kit, Human IgE Detection Kit, Immunoglobulin IgE Detection
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Recommended Products
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 102%
(IgE)
sensitivity: 0.4 ng/mL
standard curve range: 4.1-1,000 ng/mL
(IgE)
technique(s)
multiplexing: suitable
compatibility
configured for Single Plex
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.
Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX® Human IgE Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
Panel Type: Immune Response
Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX® Human IgE Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
Panel Type: Immune Response
Specificity
Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Application
- Analyte: IgE
- Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples
- Recommended Sample dilution: 1:50 diluted plasma or serum, or 50 μL cell culture supernatant
- Assay Run Time: One day
- Research Category: Inflammation & Immunology
Packaging
96 Well Plate
Components
MILLIPLEX MAP Anti-Human k and λ Light Chain, PE
MILLIPLEX MAP Human IgE Standard
MILLIPLEX MAP Human Serum Positive Control
MILLIPLEX MAP Assay Buffer
MILLIPLEX MAP Washer Buffer, 10X
Set of one 96-Well Filter Plate with 4 Sealers
MILLIPLEX MAP Human IgE Standard
MILLIPLEX MAP Human Serum Positive Control
MILLIPLEX MAP Assay Buffer
MILLIPLEX MAP Washer Buffer, 10X
Set of one 96-Well Filter Plate with 4 Sealers
Storage and Stability
Recommended storage for kit components is 2 - 8°C.
Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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Sophia Kwon et al.
Annals of the American Thoracic Society, 13(8), 1253-1261 (2016-04-21)
The World Trade Center (WTC) collapse generated caustic airborne particulates that caused chronic rhinosinusitis in exposed Fire Department of New York firefighters. Surgery was performed when symptoms remained uncontrolled despite medical management. To identify predictors of surgical intervention for chronic
José Carlos Zarzuela et al.
Frontiers in immunology, 13, 911022-911022 (2022-08-09)
Conjunctival intraepithelial lymphocytes, tear soluble molecules and commensal microbiota have important roles in the ocular mucosal immune response in healthy and diseased subjects. For the purpose of this study, the cellular and microbial populations of the conjunctiva and the lacrimal
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