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AB3226

Sigma-Aldrich

Anti-Uncoupling Protein 2 Antibody

Chemicon®, from rabbit

Synonym(s):

UCP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UCP2(7351)

Related Categories

Specificity

Uncoupling Protein 2 (UCP2), human. Human UCP2 is a 309 amino acid mitochondrial uncoupling protein (Fleury et al. 1997; Gimeon et al. 1997). It is only 59% homologous with UCP1 found in brown adipose tissues. UCP2 has wide tissue distribution in tissues (brain, kidney, liver, brown adipose tissue, heart, and muscle). UCP2 may play a critical role in energy balance, body weight, and thermoregulation (Fleury et al. 1997; Gimeon et al. 1997; Flier & Lowell 1997).

SPECIES REACTIVITIES: The antibody has not yet been tested for cross-reactivity to other species. The sequence used for immunization shows 100% homology to mouse and rat UCP2 and 92% homology with canine, porcine and bovine.

Immunogen

13 aa peptide sequence near the 3rd and 4th transmembrane domain of human UCP2 (Fleury et al. 1997; Gimeon et al. 1997). The peptide has no significant homology with UCP1, UCP3 or UCP4 (Boss et al. 1997).

Application

Detect Uncoupling Protein 2 using this Anti-Uncoupling Protein 2 Antibody validated for use in ELISA & WB.
Research Category
Metabolism
Research Sub Category
Ion & Transport Channels
Western Blotting: 1-10 μg/mL using Chemiluminescence technique.

Immunohistochemistry: Has not been tested. It is recommended that you use 2-20 μg/mL on formaldehyde fixed tissue.

ELISA: 1:10,000-1:100,000; using 50-100 ng UCP2 control peptide/well.

Optimal working dilutions must be determined by end user.

Physical form

Affinity purified immunoglobulin. Liquid in 100mM Tris, pH 7.5 with 0.2% BSA as stabilizer and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Heiko Bugger et al.
Cancer chemotherapy and pharmacology, 67(6), 1381-1388 (2010-09-03)
Doxorubicin-based chemotherapy is limited by the development of dose-dependent left ventricular dysfunction and congestive heart failure caused by reactive oxygen species (ROS). Uncoupling proteins (UCP) can inhibit mitochondrial ROS production as well as decrease myocyte damage from exogenous ROS. Prior
Rajan Singh et al.
Molecular cancer research : MCR, 14(1), 78-92 (2015-10-16)
The initiation and progression of breast cancer is a complex process that is influenced by heterogeneous cell populations within the tumor microenvironment. Although adipocytes have been shown to promote breast cancer development, adipocyte characteristics involved in this process remain poorly
Lyudmyla G Glushakova et al.
Molecular genetics and metabolism, 104(3), 255-260 (2011-08-19)
The pyruvate dehydrogenase complex (PDC) oxidizes pyruvate to acetyl CoA and is critically important in maintaining normal cellular energy homeostasis. Loss-of-function mutations in PDC give rise to congenital lactic acidosis and to progressive cellular energy failure. However, the subsequent biochemical

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