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X2753

Sigma-Aldrich

Xylanase

greener alternative

powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae

Synonyme(s) :

Pentopan Mono BG®

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About This Item

Numéro CAS:
37278-89-0
Numéro CE :
253-439-7
Numéro MDL:
MFCD00132594
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Produit recombinant

expressed in Aspergillus oryzae

Niveau de qualité

200

Forme

powder

Activité spécifique

≥2500 units/g

Caractéristiques du produit alternatif plus écologique

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Autre catégorie plus écologique

Enabling,

Température de stockage

2-8°C

Catégories apparentées

Description générale

Purified endo-β−(1→4)-xylanase from Thermomyces lanuginosus produced by submerged fermentation of a genetically modified Aspergillus oryzae microorganism
Studies show that the signal sequence found in the xylanase gene is critical for protein production in E. coli. Loss of this sequence resulted in a significant reduction in expression. [1]
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research
Xylanase catalyses the degradation of xylan into xylooligosaccharides and D-xylose. It also breaks down hemicellulose. Xylanase is used for the production of biofuels, such as ethanol and xylitol. It improves the quality of bread and juices.[2] Xylanase is used in biobleaching process and decreases environmental pollution.[3]

Application

Xylanase has been used:
  • in the isolation of hemicellulose and production of oligosaccharides[4]
  • in viscosimetric assay[5]
  • in the comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ[6]
  • in the hydrolytic degradation of xylan[7]

Autres remarques

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informations légales

A product of Novozyme Corp.
Pentopan is a registered trademark of Novozymes Corp.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000424723
0000424722
0000424723
0000424722
0000374140

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Svini Dileepa Marasinghe et al.
Microbial cell factories, 20(1), 129-129 (2021-07-10)
Xylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the β-1,4-glycosidic linkages between xylopyranosyl residues. In the present study, we focused on xynS1, the glycoside hydrolase (GH) 11 xylanase gene derived
Polarized-Light-Stimulated Enzymatic Hydrolysis of Xylan
Konieczna-Molenda A, et al.
Biotechnology Progress, 24(2), 385-388 (2008)
XYLANASES AND THEIR INDUSTRIAL APPLICATIONS: A REVIEW
Kumar D, et al.
Biochemical and Cellular Archives, 17(1), 353-360 (2017)
Gautam Anand et al.
International journal of molecular sciences, 22(8) (2021-05-01)
Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as
Xylan-binding xylanase Xyl30 from Streptomyces avermitilis: cloning, characterization, and overproduction in solid-state fermentation
Hernandez A, et al.
International Microbiology : The Official Journal of the Spanish Society for Microbiology (2008)
Afficher tous les articles scientifiques apparentés

Questions

1–7 of 7 Questions  

  1. Will this enzyme in buffer be deactivated completely at 100°C boiling for 10 mins towards end of protocol or it needs acid treatment strictly ?

    1 answer

    1. We have not tested boiling this enzyme at the end of the protocol, we have only used acid.

      Helpful?

  2. Is this enzyme powder soluble in water ? seems like particle dispersing in buffer at Ph 4.8 ?

    1 answer

    1. The active components of xylanase X2753 are readily soluble in water at concentrations that occur in normal usage. However, water solutions will be turbid because of the wheat flour used for standardization of the enzyme.

      Helpful?

  3. What does >2500U/g imply. What is range of enzyme activity for this enzyme under your protocols kindly inform.

    1 answer

    1. Each Lot is guaranteed to have a specific activity greater than or equal to 2500 Units/g. There is no maximal value specified.
      The calculated specific activity for each lot will be printed on the Certificate of Analysis found here:
      https://www.sigmaaldrich.com/product/sigma/x2753#product-documentation

      Helpful?

  4. What is the optimal pH, buffer, temperature and concentration of this product ?

    1 answer

    1. This is a product of Novozymes Corp. The manufacturer tests this material according to the following protocol and conditions:
      Temperature - 50°C
      Buffer - 0.1M Phosphate Buffer, pH 6
      Enzyme concentration - 0.007 - 0.057 units/mL
      Substrate - 0.45%w/v Azo-Wheat arabinxylan in 0.1 M Phosphate Buffer, pH 6
      Reaction - 0.100 mL enzyme solution added to 0.900 mL substrate solution
      Incubation time - 30 minutes in a water bath at 50°C
      Stop Reaction - add 5 mL of Stop Reagent: 6.66 mL 2 N HCl in 1000mL absolute Ethanol
      Incubation - 15 - 60 minutes at ambient temperature
      Centrifuge at 4000 rmp for 15 minutes
      Detection - 585 nm (manual) within 20 minutes of centrifugation

      Helpful?

  5. We have Sigma X2753 Xylanase We need to treat our sample with this. Kindly suggest a protocol at which this product will show its optimum activity.

    1 answer

    1. There is not a protocol offered specifically for this product. However, the peer-reviewed articles may be helpful:
      https://www.sigmaaldrich.com/search/x2753?focus=papers&page=1&perpage=30&sort=relevance&term=x2753&type=citation_search

      Helpful?

  6. What is the density of Product X2753, Cellulase from Aspergillus oryzae?

    1 answer

    1. We have not determined the density of this product.

      Helpful?

  7. What is the optimum pH for Cellulase from Aspergillus oryzae., Product X2753?

    1 answer

    1. Product X2753, Xylanase, has activity in the pH range of 4-6, and tolerance with temperatures up to 75 °C. The following article may be helpful: https://www.sciencedirect.com/science/article/pii/S138917230670603X#aep-section-id10

      Helpful?

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