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MAK266

Sigma-Aldrich

Kit de quantification colorimétrique/fluorimétrique des triglycérides

Triglyceride Quantification Colorimetric/Fluorometric Kit
1 of 3 reviewers received a sample product or took part in a promotion

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

Kit de dosage des TG

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Le tarif et la disponibilité ne sont pas disponibles actuellement.

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

neurological disorders; cardiovascular diseases

Température de stockage

−20°C

Catégories apparentées

Description générale

Le nouveau kit de dosage des triglycérides (réf. MAK564) est désormais disponible ! Les triglycérides (TG) sont les principaux constituants des huiles végétales, des graisses animales, des LDL et VLDL, et jouent un rôle important comme transporteurs d′acides gras ainsi que source d′énergie.[1][2] Les TG sont décomposés en acides gras et en glycérol, après quoi ces deux constituants peuvent servir de substrat pour les voies métaboliques et productrices d′énergie.[3] L'hypertriglycéridémie est associée à l'athérosclérose, aux cardiopathies et aux accidents vasculaires cérébraux ainsi qu'à la pancréatite.[4]

Adéquation

Ce produit peut être utilisé avec les échantillons de sérum, plasma et autres liquides biologiques, ainsi qu′avec les échantillons de culture cellulaire et tissulaire.

Principe

Le kit de quantification des triglycérides propose une méthode simple et sensible pour mesurer la concentration en TG de divers types d′échantillons. Dans cet essai, les TG sont convertis en acides gras libres et en glycérol. Le glycérol est alors oxydé en un produit mesurable par colorimétrie (570 nm) / fluorimétrie (lex = 535 nm/ lem = 587 nm). Ce kit permet de détecter entre 2 pmoles et 10 nmoles (plage de 2 à 10 000 mM) de triglycérides dans divers types d′échantillons. Ce kit détecte également les monoglycérides et les diglycérides.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F - closed cup

Point d'éclair (°C)

87 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
8K18K06220
8H26K06220
8H11K06220
8F20K06220
8A17K06220

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Clinical biochemistry and hematology.
The laboratory rabbit, guinea pig, hamster, and other rodents., 57-116 (2012)
Pingping Luo et al.
Acta pharmaceutica Sinica. B, 11(3), 668-679 (2021-03-30)
Alcoholic liver disease (ALD) causes insulin resistance, lipid metabolism dysfunction, and inflammation. We investigated the protective effects and direct regulating target of S-allylmercaptocysteine (SAMC) from aged garlic on liver cell injury. A chronic ethanol-fed ALD in vivo model (the NIAAA model)
Glyceroneogenesis and the triglyceride/fatty acid cycle.
Reshef L, et al.
The Journal of Biological Chemistry, 278(33), 30413-30416 (2003)
The role of triglycerides in atherosclerosis.
Talayero, Beatriz G., and Frank M. Sacks.Talayero, Beatriz G and Frank M S
Current Cardiology Reports, 13(6), 544-544 (2011)
L Vogel et al.
Journal of dairy science, 103(8), 7431-7450 (2020-06-02)
Rations including high amounts of corn silage are currently very common in dairy production. Diets with corn silage as forage source result in a low supply of essential fatty acids, such as α-linolenic acid, and may lead to low conjugated
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3 Reviews This action will navigate to reviews.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Can ethanol or isopropanol (IPA) be used instead of water for liver tissue sample preparation with the Triglyceride Quantification Colorimetric/Fluorometric Kit (MAK266)?

    1 answer

    1. Based on the guidelines, only water is an appropriate solvent for samples in this assay. Ethanol and isopropanol are not suitable solvents for this assay.

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  4. Is liver tissue extracted using RIPA buffer and without NP-40 compatible with the MAK266 Triglyceride Quantification Colorimetric/Fluorometric Kit?

    1 answer

    1. It is recommended to use NP-40 or similar non-ionic detergents instead of RIPA, due to the presence of harsh detergents in RIPA.

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Reviews

1 of 3 reviewers received a sample product or took part in a promotion

Active Filters

  1. Pittsburgh, PA
    • Review 1
    • Votes 8
    1 out of 5 stars.

    Terrible kit!

    The protocol is not well-written. I looked for several literatures who used this kit and found inconsistent data for control samples. Even the technical staffs of Sigma could not satisfy my questions and I strongly discourage other shoppers to use this.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for reaching out and sharing your review with us. We are sorry to hear about your experience with this product and is not what we would expect. Our records show your case was resolved as there were no additional follow up questions. We have provided your feedback to our Product Management team for further review.

  2. 2 Ratings-Only Reviews

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