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A propos de cet article
UNSPSC Code:
12164500
EC Number:
200-333-3
NACRES:
NA.84
Technique(s):
photometry: suitable
Usage:
sufficient for 20 assays
Storage temp.:
2-8°C
Service technique
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Laissez-nous vous aiderusage
sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (fructose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
Composants de kit seuls
Réf. du produit
Description
- fructose standard 500 mg
- glucose assay reagent 1 mL/vial
- phosphoglucose isomerase 1 mL/vial
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 3
Classe de stockage
11 - Combustible Solids
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Contenu apparenté
Instructions
Official Methods of Analysis of the AOAC, 28-28 (1995)
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| FA20-1KT | 04061833622292 |
| G3293-50ML | 04061837904998 |
| G3293-20ML | 04061837904981 |
| F2793-500MG | 04061833613313 |
