RNASEA-RO
Roche
RNase A
from bovine pancreas
Synonyme(s) :
Rnase
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About This Item
Produits recommandés
Source biologique
bovine pancreas
Niveau de qualité
Forme
dry powder
Activité spécifique
50 U/mg (+25°C, RNA as substrate)
Poids mol.
Mr = 13,700
Conditionnement
pkg of 100 mg (10109169001)
pkg of 25 mg (10109142001)
Fabricant/nom de marque
Roche
Paramètres
65 °C optimum reaction temp.
Technique(s)
flow cytometry: suitable
pH optimal
7.0-7.5
Température de stockage
2-8°C
Description générale
Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.
Application
- For analytical purposes
- Isolation of DNA (for this purpose, RNase A should be boiled)
- For cell cycle analysis by flow cytometry and propidium iodide (PI) staining
Actions biochimiques/physiologiques
RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.
Définition de l'unité
One unit is the enzyme activity that causes a decrease in absorbance of A0 to A1 within 1 minute under assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
Reconstitution
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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