10837059001

Lysozyme

from hen egg white

• Synonyme(s) : Mucopeptide N-acetylmuramoyl-hydrolase, Muramidase, lysozyme
• Numéro de classification (Commission des enzymes): 3.2.1.17 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: chicken egg white
• Niveau de qualité: 100
• Essai: ≥95%
• Forme: solid
• Activité spécifique: >23000 U/mg (Shugar units)
• Poids mol.: 14.4 kDa
• Conditionnement: pkg of 10 g
• Fabricant/nom de marque: Roche
• pH optimal: 6.0-7.0
• Absorption: 3.9 at 280 nm (10 mg enzyme/ml)

Description

Description générale

Muramidase, Mucopeptide N-acetylmuramoyl-hydrolase

Application

Lysozyme has been used for:

• Cell wall degradation
• Preparation of protoplasts
• Bacteriolysis
• Pharmacology (anti-inflammatory effect on mucous membrane; healing of tissue; protection against bleeding; normalization of mucus secretion; dissolution of pyrogens; potentiation of antibiotics)
• Food and drinks (flavor enhancer)
• Sample preparation prior to isolation of nucleic acids

Lysozyme has been used in the monitoring of transcription by real-time -PCR and sample preparation.

Actions biochimiques/physiologiques

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Définition de l'unité

One Shugar unit is that quantity of enzyme in 1 ml of a suspension of Micrococcus luteus of pH 7.0 whose initial absorbance at a wavelength of 450 nm is 0.750 for a pathlength of 10 mm which causes the absorbance to decrease at the rate of 0.001 per minute, all measurements being carried out at a temperature of +25 °C.

Notes préparatoires

Working solution: Lysozyme hydrochloride is readily soluble in water and buffer solutions but insoluble in organic solvents.
Storage conditions (working solution): -15 to -25 °C
Aqueous solutions of the hydrochloride (2 mg/ml dist. water) can be stored for several days at 2 to 8° C or at -15 to -25° C for several weeks.

Reconstitution

Prepare a stock solution at a concentration of 50 mg/ml in dist. water. Dispense into aliquots and store at -15 to -25 °C. Discard each aliquot after use.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash