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Merck
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Principaux documents

03117332001

Roche

Bovine Serum Albumin Fraction V, protease-free

from bovine serum

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.21

Source biologique

bovine

Niveau de qualité

Pureté

≥98.5% albumin basis (electrophoresis)

Forme

lyophilized

Conditionnement

pkg of 50 g

Fabricant/nom de marque

Roche

Technique(s)

ELISA: suitable

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Bovine serum albumin (BSA) is a globular, α-helical, non-glycosylated protein is produced in the liver. It consists of three homologous, structurally different domains and two sub-domains each. BSA has 17 cysteine residues cross-linked and bound into a single chain.

Application

Bovine Serum Albumin Fraction V, protease-free is used:
  • for stabilization of purified enzymes
  • for site-blocking reagent in ELISA techniques
  • as a protein standard for determination of protein concentration

Actions biochimiques/physiologiques

Bovine Serum Albumin (BSA) facilitates the transmission of drugs, hormones, and fatty acids. It is the most commonly used blocking agent in enzyme-linked immunosorbent assay (ELISA). BSA enhances the differentiation of human embryonic stem cells (hESC) and is also a chief component of cell culture media.

Qualité

Contaminants: ≤0.001% heavy metals; proteases not detectable (casein digest)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Glennys V Reynoso et al.
Methods in molecular biology (Clifton, N.J.), 2023, 287-299 (2019-06-27)
This chapter provides methods for the propagation, purification, and titration of vaccinia virus (VACV) and the highly attenuated strain-modified vaccinia Ankara (MVA). Additionally, we provide information on VACV recombinants we have used for intravital imaging with multiphoton excitation.
Jon T Sack et al.
The Journal of general physiology, 142(3), 315-324 (2013-08-14)
A family of 40 mammalian voltage-gated potassium (Kv) channels control membrane excitability in electrically excitable cells. The contribution of individual Kv channel types to electrophysiological signaling has been difficult to assign, as few selective inhibitors exist for individual Kv subunits.
Drew C Tilley et al.
The Journal of general physiology, 151(3), 292-315 (2018-11-07)
Allosteric ligands modulate protein activity by altering the energy landscape of conformational space in ligand-protein complexes. Here we investigate how ligand binding to a K+ channel's voltage sensor allosterically modulates opening of its K+-conductive pore. The tarantula venom peptide guangxitoxin-1E
Amanda B Hummon et al.
BioTechniques, 42(4), 467-470 (2007-05-11)
A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic aberrations and their
Myra E Woodworth-Hobbs et al.
The Journal of nutritional biochemistry, 25(8), 868-874 (2014-05-20)
Saturated fatty acids like palmitate contribute to muscle atrophy in a number of conditions (e.g., type II diabetes) by altering insulin signaling. Akt is a key modulator of protein balance that inhibits the FoxO transcription factors (e.g., FoxO3) which selectively

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