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G005747

HPLC Analysis of Sugars on Ascentis® Si

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] water; [B] acetonitrile; (15:85, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 10 μL

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Sucrose
    Maltose
    Maltotriose

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