Skip to Content
Merck
All Photos(1)

Key Documents

G004736

HPLC Analysis of Lysozyme on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles (Z777051)
mobile phase: 50 mM phosphate buffered saline, 200 mM Na2SO4, pH 7.0
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: 1 mg/mL in 50 mM phosphate buffered saline

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The positively-charged protein, lyzoyme (pI=11), exhibits excellent peak shape and recovery on SRT SEC-300 columns due to its highly-inert surface. Low protein recovery is usually attributed to 1) hydrophobic interactions with the stationary phase and 2) electrostatic interactions with the silica suface. SRT SEC-300 surface is hydrophilic and densely bonded to silica, therefore providing high mass recovery and good peak shape.

Legal Information

SRT is a registered trademark of Sepax Technologies

Analyte

Description
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service