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59142C40

Supelco

SUPELCOSIL LC-ABZ (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Synonym(s):

Analytical LC-ABZ Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L60

product line

SUPELCOSIL

feature

endcapped

manufacturer/tradename

SUPELCOSIL

packaging

1 ea of

extent of labeling

12.0% carbon loading

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

170 m2/g

surface coverage

surface coverage 3.4 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing practices such as using competing amines, operating at high ionic strength, and adjusting mobile phases to pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.

Application


  • Determination of active substances in binary mixture antiparasitic veterinary formulations by HPLC: This study showcases the use of the SUPELCOSIL LC-ABZ HPLC column for the precise determination of active compounds in veterinary medicine. This application is crucial in ensuring the effectiveness and safety of veterinary drugs. The study highlights the column′s capability to handle complex mixtures in pharmaceutical formulations, demonstrating its effectiveness in both academic research settings and in quality control for pharmaceutical applications (Kulik et al., 2011).

Features and Benefits

• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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J G Ufkes et al.
Pharmacy world & science : PWS, 20(2), 83-87 (1998-05-19)
To study the pharmacokinetics of dextromoramide in long-term opiate addicts on methadone maintenance therapy (MMT) a reverse-phase HPLC technique was developed to monitor dextromoramide and methadone concentrations in plasma simultaneously. After liquid-liquid extraction from plasma, dextromoramide and methadone were determined
Neil C Dias et al.
The Analyst, 128(5), 427-433 (2003-06-07)
The solvation parameter model is used to identify suitable chromatographic models for estimating the octanol-water partition coefficient for neutral compounds of varied structure by reversed-phase liquid chromatography. The stationary phase Supelcosil LC-ABZ with methanol-water mobile phases affords a series of
K Valkó et al.
Journal of pharmaceutical and biomedical analysis, 11(4-5), 361-366 (1993-04-01)
S-Adenosyl-L-methionine (SAM) and its metabolites S-adenosyl-L-homocysteine (SAH) and methyl-thioadenosine (MTA) are endogenous compounds that are heavily involved in a variety of biochemical processes, and have therefore been the target for several assays in body fluids and tissues. Reversed-phase chromatographic behaviour
Q C Li et al.
Journal of chromatographic science, 38(6), 246-254 (2000-07-13)
Nevirapine (Viramune), a dipyridiodiazepinone, is a potent and highly specific nonnucleoside inhibitor of HIV-1 reverse transcriptase. This paper describes the validation of a specific, sensitive, and stability-indicating high-performance liquid chromatography method for the assay and determination of related organic impurities
Ana B Baranda et al.
Journal of chromatography. A, 1031(1-2), 275-280 (2004-04-03)
A high-performance liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of five 1,4-dihydropyridines: amlodipine, nitrendipine, felodipine, lacidipine and lercanidipine. These drugs are widely used in the treatment of hypertension, angina pectoris and the therapy of cerebrovascular

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