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567227-U

Supelco

Discovery® BIO Wide Pore C5 (3 µm) HPLC Columns

L × I.D. 10 cm × 2.1 mm

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Pricing and availability is not currently available.

product name

Discovery® BIO Wide Pore C5 HPLC Column, 3 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel column

Quality Level

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

3.5% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

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This Item
568402-U567226-U567228-U
particle size

3 μm

particle size

5 μm

particle size

3 μm

particle size

3 μm

matrix active group

C5 (pentyl) phase

matrix active group

C5 (pentyl) phase

matrix active group

C5 (pentyl) phase

matrix active group

C5 (pentyl) phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

pore size

300 Å

pore size

300 Å

pore size

300 Å

pore size

300 Å

product line

Discovery®

product line

Discovery®

product line

Discovery®

product line

Discovery®

General description

Discovery BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC. Long-chain phases, like C8 or C18, are often too hydrophobic for proteins and can cause excessively long retention time or even irreversible binding to the column. For this reason short-chain phases, typically C3 or C4, are often used for RP-HPLC of proteins. However, these short-chain phases are susceptible to hydrolysis, resulting in short column lifetime, especially at low pH. The Discovery BIO Wide Pore C5 gives elution order similar to a conventional C4, yet has enhanced pH stability for longer column lifetime. Generally, higher efficiency separations are achievable on the Discovery BIO Wide Pore C5 because of the improvements we have made to the silica and bonded-phase chemistry.

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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