T9455

TMB Enhanced One Component HRP Membrane Substrate

• Synonym(s): HRP substrate, TMB, detection reagent, detection substrate
• UNSPSC Code: 12352204
• NACRES: NA.83

Properties

• form: solution
• Quality Level: 200
• storage temp.: 2-8°C

Description

General description

TMB Enhanced One Component HRP Membrane Substrate is a ready-to-use solution containing 3,3′,5,5′-tetramethylbenzidine (TMB) in a mildly acidic buffer. TMB is a substrate for horseradish peroxidase. The substrate system will react with sites on the membrane containing peroxidase, producing an insoluble permanent dark blue reaction product. To stop the reaction, rinse the membrane with reagent quality water. This substrate is not recommended for microwell or immunohistochemical applications.

Application

TMB Enhanced One Component HRP Membrane Substrate has been used as a colorimetric substrate for horseradish peroxidase (HRP) in an electrochemical enzymatic sandwich assay for the detection of interleukin-6 (IL-6) in biological fluids.

Caution

Stable at least one year at 2-8 °C.

Physical form

Ready-to-use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable