T5816
Tricine
BioPerformance Certified, suitable for cell culture, ≥99% (titration)
Synonym(s):
2-[[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid, N-[Tris(hydroxymethyl)methyl]glycine, N-[Tris(hydroxymethyl)methyl]glycine
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All Photos(4)
About This Item
Linear Formula:
(HOCH2)3CNHCH2CO2H
CAS Number:
Molecular Weight:
179.17
Beilstein:
1937804
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
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Quality Level
grade
BioPerformance Certified
Assay
≥99% (titration)
form
crystalline powder
storage condition
dry at room temperature
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and total aerobic microbial count, tested
color
white
useful pH range
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
cation traces
heavy metals (as Pb): ≤5 ppm
absorption
≤0.02 at 290 at 20%
application(s)
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
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General description
Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.
Application
Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.
Buffer component for separation of low molecular weight peptides.
Features and Benefits
- Suitable as a Buffer component, for Electrophoresis and Protein separation
- Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
- Tested for Endotoxins and Total Aerobic Microbial Count
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Other Notes
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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