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T1258

Sigma-Aldrich

Trizma® acetate

≥99.0% (titration)

Synonym(s):

TRIS acetate salt, Tris(hydroxymethyl)aminomethane acetate salt

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About This Item

Linear Formula:
NH2C(CH2OH)3 · CH3COOH
CAS Number:
6850-28-8
Molecular Weight:
181.19
Beilstein:
3702918
EC Number:
229-939-6
MDL number:
MFCD00038951
UNSPSC Code:
12161700
PubChem Substance ID:
24899948
NACRES:
NA.25

Quality Level

300

Assay

≥99.0% (titration)

form

crystalline powder

pH

6.0-7.0 (0.1 M in water, high purity)

solubility

water: 0.5 M, clear, colorless

SMILES string

CC(O)=O.NC(CO)(CO)CO

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

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Application

Trizma® acetate has been used:
  • in the adenosine triphosphate (ATP) assay to neutralize the pH to 7.7 of the oocyte supernatant
  • in cell wall isolation to remove proteins and membranes from the cell walls of plants
  • as a component of DamID buffer, lysis buffer for cell sorting and lysing

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S Hashimoto et al.
Molecular reproduction and development, 57(4), 353-360 (2000-11-07)
The effects of carbohydrates on meiotic maturation and ATP content of bovine oocytes under low oxygen tension (5%) were investigated. Furthermore, the developmental competence or intracellular H(2)O(2) contents of the oocytes matured under 5% or 20% O(2) was assessed. In
Máté Borsos et al.
Nature, 569(7758), 729-733 (2019-05-24)
In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic
Máté Borsos et al.
Nature, 569(7758), 729-733 (2019-05-24)
In mammals, the emergence of totipotency after fertilization involves extensive rearrangements of the spatial positioning of the genome1,2. However, the contribution of spatial genome organization to the regulation of developmental programs is unclear3. Here we generate high-resolution maps of genomic
Masanobu Yamamoto et al.
ChemSusChem, 13(17), 4400-4408 (2020-07-22)
Increases in coniferaldehyde content, a minor lignin residue, significantly improves the sustainable use of plant biomass for feed, pulping, and biorefinery without affecting plant growth and yields. Herein, different analytical methods are compared and validated to distinguish coniferaldehyde from other

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