T0665

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

• Synonym(s): Siderophilin, iron-saturated
• CAS Number: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00131337
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• biological source: human plasma
• Quality Level: 200
• description: Iron-saturated
• product line: BioReagent
• Assay: ≥97%
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: HIV, HBsAg and HCV, none detected (tested by FDA approved testing methods); endotoxin, tested
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TF(7018)

Description

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Iron content by ICP reported on a lot-specific basis.

Purity by agarose gel electrophoresis.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3