SAB4600386

Monoclonal Anti-6X His IgG, CF^™770 antibody produced in mouse

~2 mg/mL, clone 33D10.D2.G8, affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: CF^™ 770 conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: 33D10.D2.G8, monoclonal
• form: buffered aqueous solution
• concentration: ~2 mg/mL
• technique(s): indirect immunofluorescence: 1-10 μg/mL; western blot: suitable
• isotype: IgG1κ
• fluorescence: λ_ex 770 nm; λ_em 797 nm
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

This affinity purified antibody is directed against the 6X HIS epitope and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. This product is prepared by labeling high quality monoclonal mouse anti-6X His IgG(H+L) with our near-IR fluorescent dye CF770. CF770 is one of our three outstanding near-IR CF dyes developed by Biotium. Near-IR CF dyes are superior to other similar near-IR dyes, such as IRDye 800CW and the DyLight 800. Near-IR CF dyes have combined advantages in brightness, photostability, specificity and novel features ideal for in vivo imaging, near-IR Western blotting and flow cytometry.

Specificity

Binds 6X-His epitope tag

Immunogen

proteins contatining the epitope tag sequence

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 0.2% BSA.

Preparation Note

Protect from light. The antibody solution should be gently mixed before use.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable