SAB4200773

Anti-Pentraxin 3 antibody, Mouse monoclonal

clone PTX3-5, purified from hybridoma cell culture

• Synonym(s): Pentaxin-related protein PTX3, TNF alpha-induced protein 5, TSG-14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PTX3-5, monoclonal
• form: buffered aqueous solution
• mol wt: ~47 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 0.5-1 μg/mL using purified recombinant human Pentraxin 3 protein; immunoprecipitation (IP): 1-2 μg/test using lysate of HEK-293T cells over-expressing human Pentraxin 3
• isotype: IgG1
• UniProt accession no.: P26022
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PTX3(5806)

Description

General description

Anti-Pentraxin 3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the PTX3-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with recombinant human pentraxin 3 protein expressed in HEK-293 cells. Pentraxin 3 (PTX3), also called tumor necrosis factor inducible gene 14 protein (TSG-14), is a highly glycosylated secreted protein usually found as covalently-bound homooctamers. PTX3 belongs to the pentraxin subfamily, sharing the C-terminal domain with short pentraxins and contains a unique N-terminal domain. PTX3 is majorly present in monocytes, macrophages, fibroblasts, endothelial cells and adipocytes. It is an acute-phase protein mapped to human chromosome 3q25.32.

Specificity

Anti-Pentraxin 3 antibody, Mouse monoclonal recognizes both glycosylated and non-glycosylated human PTX3.

Immunogen

recombinant human Pentraxin 3 protein expressed in HEK-293 cells

Application

Anti-Pentraxin 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Pentraxin 3 (PTX3) is released in response to primary inflammatory signals, such as toll-like receptor engagement, tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β). It regulates innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 acts as a soluble pathogen recognition receptor and aids resistance against pathogens. It participates in the removal of apoptotic cells during an immune response. PTX3 associates with complement factor H and associates with synovial fluid in rheumatoid arthritis and also in conditions of sepsis. PTX3 is suppressed by pitavastatin, making it a biomarker for inflammatory cardiovascular disease.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable