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Key Documents

SAB2500080

Sigma-Aldrich

Anti-APOB antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ApoB-100, Anti-ApoB-48, Anti-Apolipoprotein B (including Ag(x) antigen), Anti-FLDB

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About This Item

MDL number:
MFCD01092562
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

goat

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

UniProt accession no.

P04114

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... APOB(338)

General description

Apolipoprotein B (APOB), a large amphipathic glycoprotein, has hydrophobic lipid-binding and hydrophilic regions. It has a low-density lipoprotein (LDL)-receptor-binding domain. The APOB gene with 29 exons produces two forms, such as apoB-48 and apoB-100 in circulating lipoproteins. This gene is located on human chromosome 2p24.1.

Immunogen

Peptide with sequence C-TDLHLRYQKDKK from the internal region of the protein sequence according to NP_000375.2.

Application

Anti-APOB antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Apolipoprotein B (APOB) participates in human lipoprotein metabolism. APOB-48 plays an essential role in the synthesis of chylomicron. APOB-100 is required for the structural component of very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), and low-density lipoprotein (LDL). APOB is essential in the molecular diagnosis of familial hypercholesterolemia (FH). The hydrophobic lipid-binding and hydrophilic regions are capable of interacting with the aqueous plasma environment.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied at 0.5 mg/mL in Tris saline with 0.02% sodium azide and 0.5% bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Amanda J Whitfield et al.
Clinical chemistry, 50(10), 1725-1732 (2004-08-17)
Plasma lipoproteins are important determinants of atherosclerosis. Apolipoprotein (apo) B is a large, amphipathic glycoprotein that plays a central role in human lipoprotein metabolism. Two forms of apoB are produced from the APOB gene by a unique posttranscriptional editing process:
Mohammed Ali Batais et al.
Medicine, 98(4), e14247-e14247 (2019-01-27)
Familial hypercholesterolemia (FH) is a monogenic dominant inherited disorder of lipid metabolism characterized by elevated low-density lipoprotein levels, and is mainly attributable to mutations in low-density lipoprotein receptor (LDLR), apolipoprotein B (APOB), and proportein convertase subtilisin/kexin type 9 (PCSK9) genes.
Guiting Zhang et al.
Molecular medicine reports, 23(2) (2020-12-24)
Patients with antiphospholipid syndrome have been identified to have higher incidence rates of atherosclerosis (AS) due to the elevated levels of anti‑β2‑glycoprotein I (β2GPI) antibody (Ab). Our previous studies revealed that the anti‑β2GPI Ab formed a stable oxidized low‑density lipoprotein (oxLDL)/β2GPI/anti‑β2GPI Ab complex

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