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Key Documents

SAB1306082

Sigma-Aldrich

ANTI-HIS TAG antibody produced in rabbit

purified immunoglobulin, buffered aqueous solution

Synonym(s):

Rabbit anti-hexa histidine tag

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

western blot: 1:1000

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Histidine has a strong association with immobilized metal ion matrices. This is because the imidazole ring has electron donor groups that form coordination bonds with the transition metal that is immobilized. Hexahistidine tag (His-tag) consists of four to six histidines that are fused genetically. It is mainly used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). His-tags are added at the amino or carboxy termini of proteins.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Yuria Jang et al.
International journal of molecular medicine, 53(1) (2023-12-08)
The binding between receptor‑activated nuclear factor‑κB (RANK) and the RANK ligand (RANKL) during osteoclast development is an important target for drugs that treat osteoporosis. The leucine‑rich repeat‑containing G‑protein‑coupled receptor 4 (LGR4) acts as a negative regulator of RANK‑RANKL that suppresses
Shuting Ding et al.
The EMBO journal, 42(6), e111858-e111858 (2022-12-24)
Phytosulfokine (PSK) is a plant pentapeptide hormone that fulfills a wide range of functions. Although PSK has frequently been reported to function in the inverse regulation of growth and defense in response to (hemi)biotrophic pathogens, the mechanisms involved remain largely
Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins? Evaluation of Two Anti-His-Tag Monoclonal Antibodies
Kristian M.Muller, et al.
Analytical Biochemistry, 54-61 (1998)
[16] Purification of Proteins Using Polyhistidine Affinity Tags
Joshua A, et al.
Methods in Enzymology (2000)
Sebastian Dorawa et al.
International journal of molecular sciences, 23(14) (2022-07-28)
We present a structural and functional analysis of the DNA polymerase of thermophilic Thermus thermophilus MAT72 phage vB_Tt72. The enzyme shows low sequence identity (<30%) to the members of the type-A family of DNA polymerases, except for two yet uncharacterized

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