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RAB0149

Sigma-Aldrich

Human EGF ELISA Kit

for serum, plasma, cell culture supernatant.

Synonym(s):

EGF ELISA Kit

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1 pg/mL
standard curve range: 0.82-200 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... EGF(1950)

General description

The Human EGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human EGF in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Human EGF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human EGF ELISA Kit has been used to examine the concentration of epidermal growth factor (EGF) in adipose derived stem cell conditioned medium (ADSC-CM).

Biochem/physiol Actions

Epidermal growth factor (EGF) is the member of EGF-like family of growth factors, which binds to EGF receptor. EGF increases proliferation and migration in bone marrow stromal cells (BMSC), that gives rise to osteoblasts, chondrocytes and adipocytes. It blocks gastric acid and pepsin secretion and enhances gastrointestinal cell proliferation. It functions as a strong mitogenic factor for various cultured cells. Mutations in this gene is associated with hypomagnesemia..

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adipose derived stem cell conditioned medium effect on proliferation phase of wound healing in Sprague Dawley rat
Tarcisia T, et al.
Medical Journal of Indonesia, 26(4), 239-245 (2018)
Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia
Groenestege WMT, et al.
The Journal of Clinical Investigation, 117(8), 2260-2267 (2007)
Human epidermal growth factor precursor: cDNA sequence, expression in vitro and gene organization
Bell GI, et al.
Nucleic Acids Research, 14(21), 8427-8446 (1986)
Lijun Wang et al.
American journal of physiology. Cell physiology, 307(8), C710-C717 (2014-08-08)
We used primary mouse corneal epithelial cells (pMCE) to examine the role of Kcnj10 in determining membrane K(+) conductance and cell membrane potential and in regulating EGF/TGFA release. Western blot, immunostaining, and RT-PCR detected the expression of Kcnj10 in mouse
Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells
Muller-Deubert S, et al.
Stem Cell Research, 24, 69-76 (2017)
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