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R9255

Sigma-Aldrich

Anti-Rat IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD00162808
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

concentration

7-14 mg/mL (by absorbance at 280 nm)

technique(s)

indirect ELISA: 1:100,000
quantitative precipitin assay: 2.5-5 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Application

Anti-Rabbit IgG (whole molecule) antibody produced in rabbit was used in to precipitate and quantify the titer of human growth hormone and anti-human growth hormone complexes at a dilution of 1:20.
Anti-Rat IgG (whole molecule) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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A M Behara et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 6(10), 2853-2858 (1992-07-01)
Implantation of autologous rodent fibroblasts genetically altered to express human growth hormone has recently been shown to be a feasible approach to the delivery of new gene products in somatic gene therapy. However, the novel gene product elicited in its
Circulating advanced glycation peptides in streptozotocin-induced diabetic rats: evidence for preferential modification of IgG light chains
Gugliucci A and Menini T
Life Sciences, 62(23), 2141-2150 (1998)
Natural variation of H3K27me3 distribution between two Arabidopsis accessions and its association with flanking transposable elements
Dong X, et al.
Genome Biology, 13(12), R117-R117 (2012)
Coline Arnould et al.
Methods in molecular biology (Clifton, N.J.), 2153, 427-438 (2020-08-26)
Among the types of damage, DNA double-strand breaks (DSBs) (provoked by various environmental stresses, but also during normal cell metabolic activity) are the most deleterious, as illustrated by the variety of human diseases associated with DSB repair defects. DSBs are
Julia J Reimer et al.
Methods in molecular biology (Clifton, N.J.), 631, 139-160 (2010-03-06)
Chromatin immunoprecipitation in combination with DNA-microarray hybridization (ChIP-chip) allows the identification of chromatin regions that are associated with modified forms of histones on a genomic scale. The ChIP-chip workflow consists of the following steps: generation of biological material, in vivo
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Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

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