O5500
Osmium tetroxide
Sealed ampule.
Synonym(s):
Osmium(VIII)-oxide, ‘Osmic acid’
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About This Item
Linear Formula:
OsO4
CAS Number:
Molecular Weight:
254.23
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
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vapor density
8.8 (vs air)
Quality Level
vapor pressure
7 mmHg ( 20 °C)
form
powder
reaction suitability
reagent type: oxidant
bp
130 °C (lit.)
mp
39.5-41 °C (lit.)
SMILES string
O=[Os](=O)(=O)=O
InChI
1S/4O.Os
InChI key
VUVGYHUDAICLFK-UHFFFAOYSA-N
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General description
Osmium tetroxide (OsO4) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.
Application
Osmium tetroxide has been used as a fixative reagent for Brevibacterium mcbrellneri, and curcumin treated human lung cancer cell lines′ observation with a scanning electron microscope. It is also suitable as a fixative for ultrastructural analysis of Toxoplasma gondii′s skeletal muscle cells.
Biochem/physiol Actions
Osmium tetroxide chemically combines with lipids making them insoluble; only 7% of the lipids can be extracted after the fixation. Therefore, the cell membranes comprising phospholipids become black and electron-dense after fixation in osmium tetroxide. It is also used to fix small amount of fat maintained in sections during paraffin processing.
Other Notes
Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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