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Key Documents

N9284

Sigma-Aldrich

Nitroreductase from Escherichia coli

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≥90% (SDS-PAGE), recombinant, expressed in E. coli

Synonym(s):

NTRA

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About This Item

MDL number:
MFCD03457727
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100 units/mL

mol wt

monomer 24000

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

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UniProt accession no.

P38489

greener alternative category

, Re-engineered

shipped in

wet ice

storage temp.

−20°C

Gene Information

Escherichia coli K12 ... nfsB(945483)

General description

Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.

Application

Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.

Biochem/physiol Actions

Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.

Physical properties

Lyophilized powder containing PBS. Does not contain BSA as excipient

Unit Definition

One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.

Preparation Note

Produced using animal component-free materials.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sébastien Jenni et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 248, 119179-119179 (2020-11-30)
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival of interest in the chemistry of diketopyrrolopyrrole (DPP) pigments. In this context, we have explored the potential of symmetrical
Structural and Mechanistic Studies of Escherichia coli Nitroreductase with the Antibiotic Nitrofurazone REVERSED BINDING ORIENTATIONS IN DIFFERENT REDOX STATES OF THE ENZYME
Race PR, et al.
The Journal of Biological Chemistry, 280(14), 13256-13264 (2005)
G M Anlezark et al.
Biochemical pharmacology, 50(5), 609-618 (1995-08-25)
A nitroreductase isolated and purified from Escherichia coli B has been demonstrated to have potential applications in ADEPT (antibody-directed enzyme prodrug therapy) by its ability in vitro to reduce dinitrobenzamides (e.g. 5-aziridinyl 2,4-dinitrobenzamide, CB 1954 and its bischloroethylamino analogue, SN
Kinetics-Based Measurement of Hypoxia in Living Cells and Animals Using an Acetoxymethyl Ester Chemiluminescent Probe
Ryan LS, et al.
ACS sensors (2019)
Mansooreh Jaberipour et al.
Biochemical pharmacology, 79(2), 102-111 (2009-08-12)
Prodrug activation gene therapy for cancer involves expressing prodrug-activating enzymes in tumour cells, so they can be selectively killed by systemically administered prodrug. For example, Escherichia colinfsB nitroreductase (E.C. 1.6.99.7)(NTR), sensitises cells to the prodrug CB1954 (5-[aziridin-1-yl]-2,4-dinitrobenzamide), which it converts
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