N3254

4-Nitrophenyl phosphate di(tris) salt

phosphatase substrate, ≥97% (enzymatic), powder

• Synonym(s): 4-Nitrophenyl phosphate bis(tris) salt
• Linear Formula: (C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂
• CAS Number: 68189-42-4
• Molecular Weight: 461.36
• Beilstein: 1578142
• EC Number: 269-198-6
• MDL number: MFCD00037009
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897633
• NACRES: NA.83

Properties

• product name: 4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
• Quality Level: 100
• Assay: ≥97% (enzymatic)
• form: powder
• solubility: water: 50 mg/mL, clear, colorless to light yellow
• storage temp.: −20°C
• SMILES string: NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
• InChI key: XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate di(tris) salt has been used:

• as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysates
• to determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)
• for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)

Biochem/physiol Actions

p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves