MAK113
ATPase/GTPase Activity Assay Kit
sufficient for 200 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 200 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer
storage temp.
2-8°C
Related Categories
General description
ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate. These enzymes play key roles in transport, signal transduction, protein biosynthesis, and cell differentiation.
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the detection of ATPase and GTPase activity and for the screening of ATPase and GTPase inhibitors.
Principle
The ATPase/GTPase Activity Assay kit provides a simple and direct procedure for measuring ATPase/GTPase activity in a microplate format. This kit uses a single reagent formulation to accurately determine enzyme activity in 30 minutes at room temperature. The malachite green reagent forms a stable dark green color with free phosphate liberated by the enzymes resulting in a colorimetric product, measured at 620 nm (600-660 nm), proportional to the enzyme activity present One unit of activity is the amount of enzyme that catalyzes the production of 1 μmole of free phosphate per minute under the assay conditions.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Meng-Yan Sun et al.
Oncology reports, 39(6), 2673-2680 (2018-04-06)
Regulator of G‑protein signaling 1 (RGS1) has been found to be a critical factor in melanoma and other malignancies. However, the mechanism involved in the RGS1‑mediated promotion of melanoma progression is not clear. We based our study on samples collected from
Meifang Li et al.
International journal of biological sciences, 15(12), 2522-2537 (2019-11-23)
Despite remarkable advancements in our understanding of breast cancer, it remains the leading cause of cancer deaths in women. Distant recurrence and metastasis is the main reason for death due to breast cancer. It is well recognized that the GATA
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Yupeng Zhao et al.
iScience, 23(9), 101494-101494 (2020-09-12)
Atlastin (ATL) is a class of dynamin-like GTPases shaping endoplasmic reticulum (ER) by mediating homotypic membrane fusion. Defect of ATLs leads to abnormal ER structure and hereditary spastic paraplegia (HSP), a neurodegenerative disease with progressive spasticity. How ATLs are regulated
Wenjing Jiang et al.
Scientific reports, 8(1), 9706-9706 (2018-06-28)
The resistance to sorafenib highly affects its clinical benefits for treating hepatocellular carcinoma (HCC). Sodium orthovanadate (SOV) is a phosphate analog that displays anti-cancer activities against various types of malignancies including HCC. The present study has demonstrated that SOV is
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