Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

M7648

Sigma-Aldrich

Anti-Myosin (Skeletal and Smooth) antibody produced in rabbit

whole antiserum

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00162703
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

animal, human

technique(s)

indirect immunofluorescence: 1:10 using cultured fibroblasts

UniProt accession no.

P35579
P35580

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYH10(4628) , MYH9(4627) , MYL9(10398)

General description

Myosin is a 500 kD protein that interacts with actin in muscle and in non-muscle cells. It is characterized with two identical heavy chains (200 kD each) and four light chains (15-20 kD each). Myosin molecules consist of two major regions; the tail (rod) and head. These molecules aggregate into filaments through the tail region and interact with actin and ATP through the head region.

Specificity

Shows a pattern of staining along stress fibers of cultured human and animal fibroblasts.

Immunogen

whole myosin (heavy and light chains) from bovine uterus.

Application

Anti-Myosin (Skeletal and Smooth) antibody produced in rabbit has been used in indirect immunofluorescence labeling.

Biochem/physiol Actions

Myosin plays a vital role in neurosensory and cortical function, membrane vesicle trafficking and determinant partitioning. Myosins with actin-stimulated ATPase activity is essential for various cellular movements. Myosins I and V mediates vesicle translocation. Myosin II with a long rod like tail domain, assembles into a bipolar thick filament and facilitate muscle contraction.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Root hair formation: F-actin-dependent tip growth is initiated by local assembly of profilin-supported F-actin meshworks accumulated within expansin-enriched bulges
Baluska F, et al.
Developmental Biology, 227(2), 618-632 (2000)
F Baluska et al.
Developmental biology, 227(2), 618-632 (2000-11-10)
Plant root hair formation is initiated when specialized elongating root epidermis cells (trichoblasts) assemble distinct domains at the plasma membrane/cell wall cell periphery complexes facing the root surface. These localities show accumulation of expansin and progressively transform into tip-growing root
Myosin: The Actin Motor Protein
Molecular Cell Biology, 10(1), 80-86 (2000)
Myosins: matching functions with motors.
Baker JP and Titus MA.
Current Opinion in Cell Biology, 10(1), 80-86 (1998)
Andrea Valigurová et al.
PloS one, 12(6), e0179709-e0179709 (2017-06-24)
Recent studies on motility of Apicomplexa concur with the so-called glideosome concept applied for apicomplexan zoites, describing a unique mechanism of substrate-dependent gliding motility facilitated by a conserved form of actomyosin motor and subpellicular microtubules. In contrast, the gregarines and
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service