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L4144

Sigma-Aldrich

Lectin from Phaseolus vulgaris (red kidney bean)

Leucoagglutinin PHA-L, lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):

Phaseolus vulgaris agglutinin, PHA

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About This Item

EC Number:
232-718-7
UNSPSC Code:
12352202
NACRES:
NA.32

Quality Level

200

product line

BioReagent

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.)
≤1.0 μg per mL leucoagglutination activity
≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

impurities

salt, free

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

suitability

leucocyte agglutination activity tested

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins, which contains four subunits with a molecular weight of 125 kDa. Leucoagglutinin PHA-L is a plant lectin, which is transported by the neurons in the central nervous system.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • it has been used as a positive control to treat cells
  • to stimulate cell culture for the analysis of lymphocyte proliferation
  • to stimulate T cells

Biochem/physiol Actions

Lectin agglutinates cells and precipitates polysaccharides and glycoproteins. It is associated with toxic allergens and hemagglutinins. Lectin exhibits anti-cancer, anti-human immunodeficiency virus (anti-HIV) and anti-microbial infection properties. Lectin inhibits mucosal atrophy, lowers type 2 diabetes and obesity, enhances nutrient absorption and regulates drug targeting.
PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.
PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides. PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin. PHA-M is the mucoprotein form. Conjugates are prepared from the corresponding purified lectins.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mumps is nowadays re-emerging despite vaccination. The contribution of T cell immunity to protection against mumps has not been clearly defined. Previously, we described a set of 41 peptides that were eluted from human leukocyte antigen (HLA) class I molecules
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Cellular substrate (CS) composed of phytohemagglutinin (PHA)-stimulated PBMNCs is required for isolating primary human immunodeficiency virus Type 1 from plasma (PHIV) during early acute infection. The transmitted founder PHIV is neutralization-sensitive and uses chemokine-receptor 5 to infect CD4-positive PBMNCs. Therefore
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Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease characterized by exocrine gland dysfunction, mainly causing sicca symptoms. B cells have a prominent role in SS, and the T follicular helper (TFH ) cells provide B cells with survival- and
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