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GE17-5157-01

HiTrap® SP Fast Flow

Cytiva 17-5157-01, pack of 5 × 5 mL

Synonym(s):

HiTrap SP

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

Pricing and availability is not currently available.

ligand

sulphopropyl

description

Ion Exchanger Type (value)

packaging

pack of 5 × 5 mL

manufacturer/tradename

Cytiva 17-5157-01

parameter

flow rate
42 psi

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

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This Item
GE17-5156-01GE17-5054-01GE17-5053-01
parameter

flow rate, 42 psi

parameter

42 psi

parameter

flow rate, 42 psi

parameter

<4 mL/min flow rate, 42 psi

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

matrix

6% cross-linked agarose

manufacturer/tradename

Cytiva 17-5157-01

manufacturer/tradename

Cytiva 17-5156-01

manufacturer/tradename

Cytiva 17-5054-01

manufacturer/tradename

Cytiva 17-5053-01

packaging

pack of 5 × 5 mL

packaging

pack of 5 × 5 mL

packaging

pack of 5 × 1 mL

packaging

pack of 5 × 1 mL

ligand

sulphopropyl

ligand

quaternary amine

ligand

sulphopropyl

ligand

quaternary amine

General description

HiTrap® SP FF is prepacked with SP Sepharose Fast Flow, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

Features and Benefits

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

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Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

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