G1163

O-Glycosidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• Synonym(s): Endo-α-N-acetylgalactosaminidase, O-Glycanase
• CAS Number: 9032-92-2
• Enzyme Commission number: 3.2.1.97 (BRENDA, IUBMB)
• EC Number: 232-888-2
• MDL number: MFCD00131003
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• conjugate: (O-linked)
• form: buffered aqueous solution
• mol wt: 180 kDa
• concentration: ≥800 units/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Biochem/physiol Actions

Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase..

Packaging

Supplied with 5× Reaction Buffer, 250 mM NaH₂PO₄ pH 5.0.

Unit Definition

One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→ΟC₆H₄NO₂) per min at 37 °C at pH 6.5.

Physical form

Solution in 50 mM sodium phosphate, pH 7.5

Analysis Note

Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves