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Clone 15 HL-60

98052918, human unknown/unspecified, Lymphoblastoid

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About This Item

UNSPSC Code:
41106514

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product name

Clone 15 HL-60, 98052918

biological source

human unknown/unspecified

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

IL-5

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Cell Line Origin

Human caucasian promyelocytic leukaemia

Cell Line Description

The clone 15 HL-60 has been derived from HL-60 cells (Sigma Catalogue number. 98070106) that had been cultured at an elevated pH of 7.6 - 7.8 for 2 months. Clone 15 HL-60 was picked from soft agar being selected for the ability to differentiate to eosinophils after treatment with butyric acid. Although 80% of the cells will exhibit this differentiation pattern initially, reversion will occur during culture leading to a higher percentage reverting to neutrophilic differentiation. Expression of IL-5 receptors and eosinophil granule proteins has been found (eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophilotactions protein (ECP) and eosinophil derived neurotoxin (EDN). In addition, chemotaxis to eosinophil specific eosinophilotactius has been described.

Application

Leukemia studies, oncogene expression, differentiation assays

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells per ml; 5% CO2; 37°C. Immediately after resuscitation use medium with 20% foetal bovine serum (FBS) to aid establishment of growing culture. Reduce FBS concentration to 10% once cells are well established in

Other Notes

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