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ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

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About This Item

UNSPSC Code:
41106514

product name

ND8/34 Cell Line from mouse, 92090904

biological source

Mouse x Rat Hybridoma nerve

description

Mouse neuroblastoma x Rat neurone hybrid

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

Cell Line Origin

Mouse neuroblastoma x Rat neurone hybrid

Cell Line Description

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

Application

Study of neuronal differentiation.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Other Notes

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Certificates of Analysis (COA)

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