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C7401

Sigma-Aldrich

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit

fractionated antiserum, lyophilized powder

Synonym(s):

Anti-Con A, Anti-Jack Bean Concanavalin A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

fractionated antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

Canavalia ensiformis

packaging

vial of ~2 mL lyophilized antiserum

technique(s)

indirect ELISA: 1:25,000

storage temp.

2-8°C

target post-translational modification

unmodified

Immunogen

Jack Bean Concanavalin A (Con A).

Application

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assays (ELISA)
  • immunochemistry
  • enhanced chemiluminescence ECL western blot analysis
  • western blot

Biochem/physiol Actions

Concanavalin A is a lectin isolated from Canavalia ensiformis or Jack bean seeds. It has potent mitogenic effect on T cells and triggers the activity of both helper and suppressor T cells exhibiting immune regulator activity. Con A triggers autophagy by binding on the cell membrane glycoprotein and is internalized to the mitochondria. It activates immune response in liver and displays anti-hepatoma effect.

Target description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Reconstitution

Reconstitute with 2 mL deionized water.

Storage and Stability

Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Legal Information

ECL is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B Fairburn et al.
Biochimie, 88(9), 1165-1174 (2006-05-13)
The stress protein gp96 exhibits a number of immunological activities, the majority of studies into which have used gp96 purified from a variety of tissues. On the basis of 1-D gel electrophoresis, the purity of these preparations has been reported
M Malerba et al.
Biochemical and biophysical research communications, 286(5), 984-990 (2001-08-31)
Several authors previously showed that the interaction between 14-3-3 proteins and plasma membrane H(+)-ATPase leads to an activated complex in which the enzyme is endowed with more favorable kinetic parameters and a more physiological pH optimum. In this paper we
Hai-Xia Wang et al.
World journal of gastroenterology, 18(2), 119-125 (2012-01-19)
As a chronic inflammatory disease of the liver, the pathogenic mechanisms of autoimmune hepatitis (AIH) have not yet been elucidated, with prognosis and diagnosis remaining unsatisfied. Currently the only viable treatments of AIH are immunosuppressant application and liver transplantation. It
Nicolas Sauvion et al.
Journal of insect physiology, 50(12), 1137-1150 (2005-01-27)
Concanavalin A (lectin from Canavalia ensiformis L., ConA) has previously been shown to act as a feeding inhibitor for Acyrthosiphon pisum, the pea aphid. In the present study a range of histochemical and biochemical techniques were used to elucidate the
Rong Wang et al.
Cell death & disease, 13(7), 626-626 (2022-07-20)
Castration-resistance of prostate cancer is one of the most challenging clinical problems. In the present study, we have performed proteomics and glycomics using LNCaP model. Growth differentiation factor-15 (GDF15) level is increased in androgen receptor (AR) inhibitor-resistant cells and the

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