Skip to Content
Merck
All Photos(1)

Key Documents

C-39221

Sigma-Aldrich

Endothelial Cell Growth Medium MV 2

SupplementPack containing all media supplements as individual vials, 1 Pack for 500 ml

Synonym(s):

Primary Cell Culture Media, Recommended with C-22221

Sign Into View Organizational & Contract Pricing

Select a Size


Select a Size

Change View

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

form

liquid

packaging

pkg of 1 kit

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Endothelial Cell Growth Medium MV 2 SupplementPack (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-22221

Application

Endothelial Cell Growth Medium MV2 was developed for the in vitro cultivation of endothelial cells from microvascular vessels, the coronary artery, and the aorta. The media are optimized for primary human cells but can also be used for bovine, porcine, murine, rat and dog endothelial cells from microvascular vessels as well as for various endothelial cell lines.Recommended for:Human Dermal Microvascular Endothelial Cells (HDMEC)Human Coronary Artery Endothelial Cells (HCAEC)Human Aortic Endothelial Cells (HAoEC)Human Pulmonary Microvascular Endothelial Cells (HPMEC)Human Dermal Lymphatic Endothelial Cells (HDLEC)Human Dermal Blood Endothelial Cells (HDBEC)Human Cardiac Microvascular Endothelial Cells (HCMEC)Human Uterine Microvascular Endothelial Cells (HUtMEC)Note: For best transfection results we recommend using PromoCell heparin-free Endothelial Cell Growth Medium MV2. Heparin, included all our other our Endothelial Cell Growth Media formulations, may reduce transfection efficiency.

Components

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Quality

All lots of PromoCell Endothelial Cell Media MV/MV2 are subjected to comprehensive quality control tests using primary human endothelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested endothelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Preparation Note

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlEpidermal Growth Factor (recombinant human): 5 ng / mlBasic Fibroblast Growth Factor (recombinant human): 10 ng / ml Insulin-like Growth Factor (Long R3 IGF): 20 ng / ml Vascular Endothelial Growth Factor 165 (recombinant human): 0.5 ng / mlAscorbic Acid: 1 µg / mlHydrocortisone: 0.2 µg / ml

Storage and Stability

Store the SupplementPack at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.Note: The SupplementPack is delivered thawed and can be frozen after arrival without losing any activity.

Subculture Routine

Click here for more information.

recommended

Product No.
Description
Pricing

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Protocols

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Cell culture protocol: the endothelial cell transwell migration and invasion assay used to study angiogenesis and cancer cell metastasis. Explore over 350 PromoCell products.

Related Content

Cell culture protocol: the endothelial tube formation assay to study angiogenesis using HUVECs and other endothelial cell types. Explore over 350 PromoCell products.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service