As listed in the "Properties" section of the product detail page, this material will have 8-16 moles of biotin per mole of BSA. The exact result of biotin moles will be listed on the lot specific certificate of analysis.
A8549
Albumin, biotin labeled bovine
lyophilized powder
Synonym(s):
Biotin labeled albumin
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Pricing and availability is not currently available.
Recommended Products
biological source
bovine
Quality Level
Assay
≥80% protein basis (biuret)
form
lyophilized powder
concentration
≥97% protein in anhydrous DMSO (spectralphotometric; calc. on anhydrous substance)
≥98% (Assay of Albumin (Agarose gel electrophoresis))
extent of labeling
8-16 mol biotin per mol albumin
technique(s)
bioconjugation: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
Biotin labeled bovine albumin has been used as a component in myosin V processivity experiments.[1] It has also been used to provide biotin tags for neutravidin coating and also to suppress nonspecific cell adhesion during preparation of tension gauge tether (TGT) surface.[2]
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues.
Biotin-conjugated bovine serum albumin binds selectively to different vascular subsets in lymphoid tissues, as demonstrated by both fluorescent and enzymohistochemical techniques, and thus offers a histochemical probe for identification of select microvascular endothelia in lymphoid tissues. In spleen, biotin-BSA binds to red pulp sinuses, but not white pulp vessels (including the central arteriole) nor the marginal sinus. In the thymus, prominent binding was restricted to the capillaries of the corticomedullary area.
Physical form
Contains sodium citrate buffer salts
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Integrin Molecular Tension within Motile Focal Adhesions
Wang X, et al.
Biophysical Journal, 109, 2259-2267 (2019)
Photothermal detection of individual gold nanoparticles: perspectives for high-throughput screening.
Florian Kulzer et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 9(12), 1761-1766 (2008-07-31)
We use photothermal microscopy to detect and image individual gold nanoparticles that are either embedded in a polymer film or immobilized in an aqueous environment. Reducing the numerical aperture of the detection optics allows us to achieve a 200-fold-enlarged detection
Tilting and Wobble of Myosin V by High-Speed Single-Molecule Polarized Fluorescence Microscopy
Beausang JF, et al.
Biophysical Journal, 104, 1263-1273 (2019)
Taikopaul Kaneko et al.
Science advances, 6(4), eaax7413-eaax7413 (2020-02-06)
Kinesin is a motor protein that plays important roles in a variety of cellular functions. In vivo, multiple kinesin molecules are bound to cargo and work as a team to produce larger forces or higher speeds than a single kinesin.
Parastoo Maleki et al.
Molecular genetics and genomics : MGG, 292(3), 483-498 (2017-02-06)
In this article, we summarize the knowledge and best practices learned from bulk and single-molecule measurements to address some of the frequently experienced difficulties in single-molecule Förster resonance energy transfer (smFRET) measurements on G-quadruplex (GQ) structures. The number of studies
-
How many biotin moles are there per mole of BSA of Product A8549, Albumin, biotin labeled bovine?
1 answer-
Helpful?
-
-
What is the molecular weight for biotin labeled bovine(Product A8549, Albumin)?
1 answer-
In order to determine the molecular weight of this product, review the lot specific Certificate of Analysis for the Degree of Substitution (this provides the moles of biotin per mol of albumin). For lot SLCM9516, the Degree of Substitution is 8 mols biotin per mol of albumin. 8 x 244.31 (MW of biotin) + 66,430 (MW of BSA) = 68,384
Helpful?
-
-
What is the method of preparation for Product A8549, Albumin, biotin labeled bovine?
1 answer-
The method of preparation of this product is considered proprietary by Sigma-Aldrich.
Helpful?
-
-
What is the linker between the BSA and the biotin for Product A8549, Albumin, biotin labeled bovine?
1 answer-
There is no linker or "spacer". The link between the BSA and the biotin is an amide bond connecting an amino group on the BSA to the carboxy group on the biotin.
Helpful?
-
-
Do you have the amino acid sequence for bovine serum albumin?
1 answer-
Bovine Serum Albumin is produced as a protein of 607 amino acids. The signal peptide (amino acids 1-18) and the propeptide (amino acids 19-22) are cleaved to make the mature form. The mature form of BSA is 583 amino acids (amino acids 25-607, Uniprot P02769). The sequence for the mature protein is below:DTHKSEIAHRFKDLGEEHFKGLVLIAFSQYLQQCPFDEHVKLVNELTEFAKTCVADESHAGCEKSLHTLFGDELCKVASLRETYGDMADCCEKQEPERNECFLSHKDDSPDLPKLKPDPNTLCDEFKADEKKFWGKYLYEIARRHPYFYAPELLYYANKYNGVFQECCQAEDKGACLLPKIETMREKVLTSSARQRLRCASIQKFGERALKAWSVARLSQKFPKAEFVEVTKLVTDLTKVHKECCHGDLLECADDRADLAKYICDNQDTISSKLKECCDKPLLEKSHCIAEVEKDAIPENLPPLTADFAEDKDVCKNYQEAKDAFLGSFLYEYSRRHPEYAVSVLLRLAKEYEATLEECCAKDDPHACYSTVFDKLKHLVDEPQNLIKQNCDQFEKLGEYGFQNALIVRYTRKVPQVSTPTLVEVSRSLGKVGTRCCTKPESERMPCTEDYLSLILNRLCVLHEKTPVSEKVTKCCTESLVNRRPCFSALTPDETYVPKAFDEKLFTFHADICTLPDTEKQIKKQTALVELLKHKPKATEEQLKTVMENFVAFVDKCCAADDKEACFAVEGPKLVVSTQTALA
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the solution stability of Product A8549, Albumin, biotin labeled bovine?
1 answer-
Sigma Aldrich has not tested the stability of this product in solution. It is recommended to prepare fresh solutions.
Helpful?
-
-
What is Product A8549, Albumin, biotin labeled bovine, soluble in?
1 answer-
This product is soluble in water. This product contains sodium citrate buffer salts, so addition of a buffer is probably not necessary; just adding water is sufficient.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
