A7732

Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal

clone EA-53, purified from hybridoma cell culture

• Synonym(s): Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582, Anti-1110008F24Rik, Sarcomeric Alpha Actinin Antibody, Sarcomeric Alpha Actinin Antibody - Anti-α-Actinin (Sarcomeric) antibody, Mouse monoclonal
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: EA-53, monoclonal
• form: buffered aqueous solution
• species reactivity: cat, rat, fish, rabbit, sheep, bovine, goat, canine, pig, mouse, human, hamster, snake, frog, lizard, chicken
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using protease-digested sections of human tongue.; western blot: 2-4 μg/mL using rat skeletal muscle extract
• isotype: IgG1
• UniProt accession no.: Q08043
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTN2(88) ; mouse ... Actn2(11472) ; rabbit ... ACTN3(100009544) ; rat ... Actn2(291245)

Description

General description

α-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. The gene encoding α-actinin is mapped to the human chromosome 11q13.2. α-Actinin belongs to the spectrin protein superfamily.
α-Actinin dimerizes in an antiparallel fashion via interaction of the central rod domains. Four isoforms are found in human and mice. α-actinin-1 is ubiquitously expressed and located primarily in focal adhesions, whereas α-actinin-4 is present in certain membrane ruffles and seems to play a role in endocytosis and tumor cell motility.
ACTN1 (α-actinin-1) and ACTN4 (α-actinin-4) encodes non-muscle proteins, while ACTN2 (α-actinin-2) and ACTN3 (α-actinin-3) encodes muscle proteins.
Monoclonal Anti-α-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish α-actinin.

Specificity

Monoclonal Anti-α-Actinin (Sarcomeric) specifically recognizes α-skeletal muscle actinin and α-cardiac muscle actinin.

Immunogen

Purified rabbit skeletal α actinin.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunofluorescence (2 papers)
Immunohistochemistry (1 paper)

Mouse Monoclonal Anti-alpha-Actinin (Sarcomeric) antibody has been used for immunocytochemical (1:800) and immunohistochemical (1:100) applications.

Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody.

Biochem/physiol Actions

α-Actinin maintains the structure of cytoskeletal organization and regulates muscle contraction. α-Actinin functions as an actin crosslinker and promotes cell migration. Mutations in the α-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves