A1595

Bovine Serum Albumin solution

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

• Synonym(s): Albumin solution from bovine serum, BSA solution
• CAS Number: 9048-46-8
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.27

Properties

• biological source: bovine
• Quality Level: 300
• sterility: sterile-filtered
• product line: BioReagent
• Assay: 90-120 mg/mL protein basis (biuret)
• form: solution
• purified by: heat shock fractionation
• contains: does not contain stabilizer or preservative
• origin: USA origin
• concentration: 10% in DPBS
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.05% fatty acid; ≤200 EU/mL endotoxin
• pH: 7.1-7.7
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.

BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.

Features and Benefits

• Fatty acid free / tested to be low in fatty acid content
• Low endotoxin
• Sterile-filtered

Preparation Note

Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves