84655
Schiff′s reagent for aldehydes
for microscopy
Synonym(s):
Fuchsin sulfurous acid
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About This Item
UNSPSC Code:
12352300
NACRES:
NA.21
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General description
Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.
Application
Schiff′s reagent for aldehydes has been used:
- in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water
- to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants
- in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B - Met. Corr. 1
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
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