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81253

Sigma-Aldrich

Poly(ethylene glycol)

BioUltra, for molecular biology, 6,000

Synonym(s):

Polyethylene glycol, PEG

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About This Item

Linear Formula:
H(OCH2CH2)nOH
CAS Number:
25322-68-3
MDL number:
MFCD00081839
UNSPSC Code:
12352104
PubChem Substance ID:
24887713
NACRES:
NA.26

grade

for molecular biology

Quality Level

100
200

product line

BioUltra

form

solid

mol wt

Mr 5000-7000

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

5.5-7.0 (25 °C, 50 mg/mL in H2O)

solubility

H2O: 50 mg/mL at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤200 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

50 mg/mL in H2O

UV absorption

λ: 260 nm Amax: 0.15
λ: 280 nm Amax: 0.10

suitability

suitable for (molecular biology applications)

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Application

Poly(ethylene glycol) has been used for concentrating HIV (human immunodeficiency virus). It has been used in the solution prepared to study CD (circular dichroism) spectra of nisin.

Other Notes

Preparing phage and plasmid DNA - precipitation of λ phage; Fractionation of DNA fragments

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nisin antimicrobial activity and structural characteristics at hydrophobic surfaces coated with the PEO?PPO?PEO triblock surfactant Pluronic? F108.
Tai Y-C, et al.
Journal of Colloid and Interface Science, 322, 104-111 (2008)
Peini Hou et al.
Structure (London, England : 1993), 27(5), 837-845 (2019-03-05)
The evolutionarily conserved Trithorax group protein Ash1 is a SET domain histone methyltransferase that mono- and dimethylates lysine 36 of histone H3 (H3K36). Ash1 forms a complex with Mrg15 and Nurf55, and the binding of Mrg15 greatly stimulates the catalytic
Fractionation of DNA fragments by polyethylene glycol induced precipitation.
J T Lis
Methods in enzymology, 65(1), 347-353 (1980-01-01)
Smoothened Agonist Reduces Human Immunodeficiency Virus Type-1-Induced Blood-Brain Barrier Breakdown in Humanized Mice.
Singh VB, et al.
Scientific Reports, 6, doi: 10-doi: 10 (2016)
Benjamin J Burwitz et al.
Journal of immunology (Baltimore, Md. : 1950), 204(8), 2169-2176 (2020-03-13)
Currently 247 million people are living with chronic hepatitis B virus infection (CHB), and the development of novel curative treatments is urgently needed. Immunotherapy is an attractive approach to treat CHB, yet therapeutic approaches to augment the endogenous hepatitis B
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