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60128

Sigma-Aldrich

Potassium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Synonym(s):

KCl

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About This Item

Linear Formula:
KCl
CAS Number:
7447-40-7
Molecular Weight:
74.55
EC Number:
231-211-8
MDL number:
MFCD00011360
UNSPSC Code:
12352302
PubChem Substance ID:
57651723
NACRES:
NA.26

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (AT)

form

solid

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.2% loss on drying, 110 °C

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

770 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.98 g/mL at 25 °C (lit.)

anion traces

bromide (Br-): ≤700 mg/kg
iodide (I-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤2 mg/kg
Li: ≤5 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

SMILES string

[Cl-].[K+]

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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Application

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Other Notes

In DpnI/MboI assay for detection of methylation changes in DNA replication

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M.L. DePamphilis
Methods in Enzymology, 262, 653-653 (1995)
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Bacterial nucleoid-associated proteins, such as H-NS-like proteins in Enterobacteriaceae, are abundant DNA-binding proteins that function in chromosomal DNA organization and gene transcription regulation. The Mycobacterium tuberculosis Lsr2 protein has been proposed to be the first identified H-NS analogue in Gram-positive
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