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T7293

Millipore

Casein Peptone

Enzymatic digest of casein from bovine milk, 11.0-16.0% total nitrogen, suitable for biotechnology and microbiology

Synonym(s):

Peptone from casein, Peptone from casein, Pancreatic digest of casein., Tryptone

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About This Item

CAS Number:
91079-40-2
EC Number:
293-428-4
UNSPSC Code:
41106212
NACRES:
NA.74

product name

Tryptone, Microbiologically tested.

biological source

bovine milk

Quality Level

200

form

powder

quality

Microbiologically tested.

packaging

bottle of 1 kg
bottle of 250 g

nitrogen analysis

11.0-16.0% total

loss

≤6% loss on drying

color

light yellow

pH

6.9-7.4

application(s)

food and beverages
microbiology

suitability

Escherichia coli
Haemophilus spp.
Lactobacillus spp.
Streptococcus spp.
coliforms

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General description

Tryptone is a mixture of peptides that is commonly used as a nutrient source in microbiological culture media. It is derived from soybean casein digest and contains a complex mixture of amino acids and peptides, including tryptophan. Tryptone is often used in combination with other ingredients, such as yeast extract and agar, to provide a nutrient-rich environment for the growth of microorganisms. It is particularly useful for culturing fastidious bacteria, which require a more complex nutrient source than simple sugars or amino acids. Tryptone is also used as a component of some enrichment broths and is a common ingredient in commercial media formulations.

Application

Tryptone has a wide range of applications in microbiology, including:
  • Growth media: Tryptone is frequently used as a source of nutrients in growth media for bacteria, yeast, and other microorganisms. It can be used alone or in combination with other ingredients, such as agar, glucose, and salts, to provide a complete nutrient source for microbial growth
  • Enrichment media: Tryptone is often added to enrichment broths to enhance the growth of slow-growing or stressed bacteria in clinical or environmental samples. This is particularly useful for samples where the target microbe is present in low numbers or is difficult to isolate
  • Production of recombinant proteins: Tryptone is a component of many bacterial growth media used to produce recombinant proteins. It provides a rich source of amino acids and peptides, which are needed for protein biosynthesis
  • It is also used in microbiological testing to determine the minimum inhibitory concentration (MIC) of antimicrobial agents against test organisms.Overall, the diverse and versatile properties of Tryptone make it a valuable tool in various microbiological applications

Preparation Note

Pancreatic digest of casein.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000378916
0000354107
0000354107
0000318180
0000306811

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Joseph A Bentil et al.
World journal of microbiology & biotechnology, 35(6), 82-82 (2019-05-28)
In developing countries, local enzyme production can help decrease the dependency of imported enzymes for bioconversion of e.g. cellulosic feedstocks, but the use of conventional nitrogen sources contributes significantly to such enzyme production cost. Use of local resources is therefore
Tarja Kaijalainen et al.
Journal of clinical microbiology, 42(1), 412-414 (2004-01-13)
In STGG (skim milk, tryptone, glucose, glycerol) medium at -80 degrees C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in nasopharyngeal swabs for at least 1.5 years.
Samantha B Kasloff et al.
Scientific reports, 11(1), 984-984 (2021-01-15)
The spread of COVID-19 in healthcare settings is concerning, with healthcare workers representing a disproportionately high percentage of confirmed cases. Although SARS-CoV-2 virus has been found to persist on surfaces for a number of days, the extent and duration of
S Oh et al.
Applied and environmental microbiology, 61(11), 3809-3814 (1995-11-01)
This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology.
Frederick K Balagaddé et al.
Science (New York, N.Y.), 309(5731), 137-140 (2005-07-05)
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying

Articles

Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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