Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

C2734

Sigma-Aldrich

5-Carboxytetramethylrhodamine

≥95% purity (HPLC), solid

Synonym(s):

5-TAMRA

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
91809-66-4
Molecular Weight:
430.45
MDL number:
MFCD01073638
UNSPSC Code:
12171500
NACRES:
NA.47

product name

5-Carboxytetramethylrhodamine,

Assay

≥95% (HPLC)

Quality Level

100

form

solid

solubility

water: slightly soluble

ε (extinction coefficient)

≥85000-97000 at 539-545 nm in methanol

fluorescence

λex ~543 nm; λem ~570 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

YMZMTOFQCVHHFB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
MKCT6671
MKCR5570
MKCQ1970
MKCM5246
MKCH9058

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Limary M Cancel et al.
Scientific reports, 8(1), 9265-9265 (2018-06-20)
We investigated the effects of direct current stimulation (DCS) on fluid and solute transport across endothelial cell (EC) monolayers in vitro. Our motivation was transcranial direct current stimulation (tDCS) that has been investigated for treatment of neuropsychiatric disorders, to enhance
Moontaha Mahbub et al.
Nature plants, 6(9), 1179-1191 (2020-09-09)
The thylakoid membranes of cyanobacteria form a complex intracellular membrane system with a distinctive proteome. The sites of biogenesis of thylakoid proteins remain uncertain, as do the signals that direct thylakoid membrane-integral proteins to the thylakoids rather than to the
Giovanni Luongo et al.
ACS applied materials & interfaces, 9(46), 40059-40069 (2017-10-13)
Iron oxide nanostructures have been widely developed for biomedical applications because of their magnetic properties and biocompatibility. In clinical applications, stabilization of these nanostructures against aggregation and nonspecific interactions is typically achieved using weakly anchored polysaccharides, with better-defined and more
Dustin J Paul et al.
Nucleic acids research, 37(17), 5894-5907 (2009-09-04)
Herein, we report the identification of RNA hairpin loops that bind derivatives of kanamycin A, tobramycin, neamine, and neomycin B via two-dimensional combinatorial screening, a method that screens chemical and RNA spaces simultaneously. An arrayed aminoglycoside library was probed for
High-selectivity neural probe based on a Fabry-Perot optical filter and a CMOS silicon photodiodes array at visible wavelengths
Pimenta S, et al.
Journal of Biomedical Optics, 23(10), 105004-105004 (2018)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service