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95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

Synonym(s):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

Empirical Formula (Hill Notation):
C37H27N3Na2O9S3
CAS Number:
28983-56-4
Molecular Weight:
799.80
Colour Index Number:
42780
EC Number:
249-352-9
MDL number:
MFCD00058509
UNSPSC Code:
12171500
PubChem Substance ID:
329770433
NACRES:
MA.02

grade

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Quality Level

200

form

powder or crystals

technique(s)

microbe id | staining: suitable

pH

9.4-14

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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General description

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Application

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

>572.0 °F - Pensky-Martens closed cup

Flash Point(C)

> 300 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK8203
BCCB9353
BCBV2236
BCBR0621V
BCBM0641V

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M Safaríková et al.
Chemosphere, 59(6), 831-835 (2005-04-07)
Brewer's yeast (bottom yeast, Saccharomyces cerevisiae subsp. uvarum) cells were magnetically modified using water based magnetic fluid stabilized with perchloric acid. Magnetically modified yeast cells efficiently adsorbed various water soluble dyes. The dyes adsorption can be described by the Langmuir
Lulu Fan et al.
Journal of hazardous materials, 215-216, 272-279 (2012-03-21)
A novel magnetic composite bioadsorbent composed of magnetic chitosan and graphene oxide (MCGO) was prepared as the magnetic adsorbent. The morphology, chemical structure and magnetic property of the MCGO were characterized by Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD)
Yu-Lin Kuo et al.
Journal of hazardous materials, 190(1-3), 938-944 (2011-05-11)
An optimal condition applied to the Taguchi method with an L(9) orthogonal array for preparing a visible-light driven nitrogen-modified TiO(2) (N-TiO(2)) photocatalyst by a simple hydrolysis method has been examined for material characteristics and a photodecolorization test of methyl blue
Lulu Fan et al.
International journal of biological macromolecules, 50(2), 444-450 (2012-01-10)
A novel nano-adsorbent, β-cyclodextrin-chitosan (CDC) modified Fe(3)O(4) nanoparticles (CDCM) is fabricated for removal of methyl blue (MB) from aqueous solution by grafting CDC onto the magnetite surface. The characteristics results of FTIR, SEM and XRD show that CDC is grafted
Sebastian Thaler et al.
Retina (Philadelphia, Pa.), 29(9), 1257-1265 (2009-11-26)
To investigate the biocompatibility of methyl blue and aniline blue as vital dyes for vitreoretinal surgery in an in vivo rat model and to evaluate the effect of these dyes on retinal structure and function. Adult Brown-Norway rats received intravitreal
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