PWOSUP-RO
Roche
Pwo SuperYield DNA Polymerase
suitable for PCR, Difficult Templates/Specialty Enzymes PCR, hotstart: no, dNTPs included: no
About This Item
Recommended Products
biological source
(pyrococcus woesei)
Quality Level
form
liquid
usage
sufficient for ≤200 reactions (04340850001)
sufficient for 200 reactions
sufficient for ≤40 reactions (04340868001)
sufficient for 40 reactions
specific activity
5 U/μL
mol wt
~ 90 kDa
feature
Difficult Templates/Specialty Enzymes PCR
High Fidelity PCR
dNTPs included: no
hotstart: no
packaging
pkg of 100 U (04340868001)
pkg of 500 U (04340850001 [2 x 250 U])
manufacturer/tradename
Roche
concentration
50 U/mL
Related Categories
1 of 4
This Item | SAB5701170 | SAB4502491 | SAB4200576 |
---|---|---|---|
100 | 100 | - | - |
unconjugated | - | unconjugated | unconjugated |
affinity isolated antibody | - | affinity isolated antibody | affinity isolated antibody |
Prestige Antibodies® Powered by Atlas Antibodies | - | - | - |
polyclonal | monoclonal | polyclonal | polyclonal |
General description
Pwo SuperYield DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.
The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.
PCR products are blunt-ended directly useable for blunt-end ligation.
Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.
Specificity
Application
- Pwo Super Yield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for: High fidelity PCR[1]
- Site-directed mutagenesis
- Cloning[2]
- Gene expression
- Study of allelic polymorphism in individual RNA transcripts
- Characterization of rare mutations in tissue
- Characterization of the allelic stage of single cells or single DNA molecules[3]
Features and Benefits
- Higher yield and 18-fold higher fidelity
- High performance with difficult templates.
- Reduce working steps in cloning.
Packaging
Quality
Unit Definition
Unit Assay: Incubation buffer for assay on activated DNA
20 mM Tris-HCl, pH 8.8 (20 °C), 50 mM KCl, 2.5 mM MgCl2, 10 mM 2-mercaptoethanol, 0.2 mM of each dATP, dCTP, dGTP, dTTP.
Incubation procedure
12.5 mg activated calf thymus DNA and 0.1 mCi [α-32P]dCTP are incubated with 0.01 to 0.1 U Pwo SuperYield DNA Polymerase in 50 μl incubation buffer with a paraffin-oil overlay at +70 °C for 30 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation followed by scintillation counting.
Volume Activity: 5 U/μl
Other Notes
Pwo DNA Polymerase accepts modified nucleotides like digoxigenin-dUTP, biotin-dUTP, or fluorescein-dUTP. Thus, it can add these nucleotides to DNA during PCR. These nonradioactively labeled products can be used as a hybridization probe in many applications.
Legal Information
Kit Components Only
- Pwo SuperYield DNA Polymerase 5 U/μl
- PCR buffer, containing 15 mM MgSO4 10x concentrated
- GC-RICH Solution 5x concentrated
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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