ABN78C3
Anti-NeuN (rabbit) Antibody, Cy3 Conjugate
from rabbit, CY3 conjugate
Synonym(s):
NEUronal Nuclei, Neuronal Nuclei
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
conjugate
CY3 conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
technique(s)
immunocytochemistry: suitable
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
General description
ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Specificity
The unconjugated rabbit Anti-NeuN (Catalog No. ABN78) has been shown to work in human.
This antibody recognizes NeuN at the N-terminus.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to mouse NeuN.
Application
Anti-NeuN (rabbit) Antibody, Cy3 Conjugate is an antibody against NeuN (rabbit) for use in IC.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
Quality
Evaluated by Immunocytochemistry in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in rat E18 cortex cells.
Target description
The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates.
Physical form
Affinity purified
Purified rabbit polyclonal conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Storage and Stability
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Rat E18 cortex cells
Rat E18 cortex cells
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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