ABE262
Anti-HDAC4 Antibody
serum, from rabbit
Synonym(s):
Histone deacetylase 4, HD4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... HDAC4(9759)
General description
Histone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Transcription is controlled in part by the dynamic acetylation and deacetylation of histone proteins. Histone deacetylases (HDACs) are recruited to specific promoters or chromosomal domains by association with DNA-binding proteins. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors MEF2A, MEF2C, and MEF2D. HDAC4 also interacts with the breast cancer associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner.
Specificity
This antibody recognizes HDAC4 at the C-terminus.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human HDAC4.
Application
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HDAC4 in HeLa and A431 cells.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in ChIP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Laboratory of Dr. Edward Seto, Moffitt Cancer Center)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
Histones
Chromatin Biology
Use Anti-HDAC4 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC, IP, ChIP to detect HDAC4 also known as Histone deacetylase 4, HD4.
Quality
Evaluated by Western Blot in HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected HDAC4 on 10 µg of HepG2 cell lysate.
Target description
~125 kDa observed
Linkage
Replaces: 04-232
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HepG2 cell lysate
HepG2 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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