ABE1310

Anti-ALKBH1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ALKBH1, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA lyase ABH1, DNA oxidative demethylase ALKBH1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): immunohistochemistry: suitable; western blot: suitable
• NCBI accession no.: NP_006011
• UniProt accession no.: Q13686
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ALKBH1(8846)

Description

General description

ALKBH1 is a DNA lyase that is critical for DNA repair and specifically repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. ALKBH1 can also cleave both single-stranded and double-stranded DNA at basic sites, and ALKBH1 can induce double stranded breaks at basic sites. Finally, ALKBH1 plays a role in placental trophoblast lineage differentiation during development, and ALKBH1 may also play a role in neuronal development and migration. ALKBH1 is widely expressed and localizes to mitochondria and nucleus. The nuclear localization of ALKBH1 appears to be as part of fusion proteins with nuclear targeted proteins or when the first 26 N-terminal residues are removed.

Specificity

Human AlkB homolog 1 is a mitochondrial protein that demethylates 3-methylcytosine in DNA and RNA (Westbye, M.P., et al. (2008). JBC. 283:25046-25056).

Immunogen

Epitope: Around the Substrate binding region

KLH-conjugated linear peptide corresponding to the substrate binding region of human ALKBH1.

Application

Anti-ALKBH1 Antibody is an antibody against ALKBH1 for use in Immunohistochemistry and Immunocytochemistry.

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ALKBH1 in human pancreas and human kidney tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected ALKBH1 in 10 µg of human skeletal muscle tissue lysate.

Target description

~45 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable