AB5830
Anti-8-Hydroxydeoxyguanosine Antibody
serum, Chemicon®
Synonym(s):
8-OHdG, 8-hydroxy-2′-deoxyguanosine
About This Item
Recommended Products
biological source
goat
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
shipped in
dry ice
target post-translational modification
unmodified
1 of 4
This Item | PHR1467 | PHR3228 | 31679 |
---|---|---|---|
neat | neat | - | neat |
200 | 300 | 300 | 100 |
analytical standard | certified reference material, pharmaceutical secondary standard | certified reference material, pharmaceutical secondary standard | analytical standard |
2-8°C | 2-8°C | 2-30°C | - |
limited shelf life, expiry date on the label | - | - | limited shelf life, expiry date on the label |
General description
8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.
CHEMICON′s new goat antiserum to 8-hydroxyguanosine is now available. This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides. This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.
Specificity
Immunogen
Application
Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% NGS in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
Optimal working dilutions must be determined by the end user.
Analysis Note
Skin, staining is limited to the nucleus.
Other Notes
Legal Information
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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