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34115-M

Millipore

N-Ethylmaleimide

Sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells.

Synonym(s):

N-Ethylmaleimide, NEM

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About This Item

Empirical Formula (Hill Notation):
C6H7NO2
CAS Number:
Molecular Weight:
125.13
MDL number:
UNSPSC Code:
12352111

Pricing and availability is not currently available.

Quality Level

Assay

≥98% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

solubility

ethanol: 5 mg/mL

storage temp.

10-30°C

InChI

1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3

InChI key

HDFGOPSGAURCEO-UHFFFAOYSA-N

General description

Sulfhydryl alkylating reagent that inhibits H+-ATPase and suppresses the short circuit current (IC50 = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA2 in endothelial cells.
Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg2+ and Ca2+/Mg2+-stimulated DNA fragmentation in rat liver nuclei.

Biochem/physiol Actions

Primary Target
H+-ATPase
Target IC50: 22 µM in suppressing short circuit current

Warning

Toxicity: Corrosive (L)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbonesCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

163.2 °F - closed cup

Flash Point(C)

72.9 °C - closed cup


Certificates of Analysis (COA)

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